After my 3 sessions of plasma dilution by membrane plasmapheresis (see previous posts), the last one been on December 23, I had some blood tests done on January 25, 2021. All the results are posted below. I am still waiting on the epigenetic age results by mydnage.com.
First, the results:
| Biomarker | 15/06/2020 | 25/11/2020 | 08/12/2020 | 17/12/2020 | 25/01/2021 |
|---|---|---|---|---|---|
| Sex | Male | ||||
| Chronological age | 34 | 34 | 34 | 34 | 34 |
| Epigenetic age (mydnage.com, derivated from Horvath Original 2013) | 30 | Sample lost by UPS | Waiting for data | ||
| Levine’s Phenotypical age clock (blood) | 22 | 26 | |||
| IGF-1 | 146 ug/L | 179 ug/L | |||
| Haemoglobin | 143 g/L | 155 | |||
| Haematocrit | 0.416 L/L | 0.467 L/L | |||
| Red Cell Count | 4.57 x10^12/L | 5.09 x10^12/L | |||
| MCV | 91.2 fl | 91.8 fl | |||
| MCH | 31.3 pg | 30.4 pg | |||
| MCHC | 343 g/L | 331 g/L | |||
| RDW | 13.2% | 13.4% | |||
| White Cell Count | 4.51 x10^9/L | 4.38 x10^9/L | |||
| Neutrophils | 1.34 x10^9/L | 1.16 x10^9/L | |||
| Lymphocytes | 2.69 x10^9/L | 2.75 x10^9/L | |||
| Monocytes | 0.29 x10^9/L | 0.27 x10^9/L | |||
| Eosinophils | 0.06 x10^9/L | 0.07 x10^9/L | |||
| Basophils | 0.11 x10^9/L | 0.1 x10^9/L | |||
| Platelet Count | 191 x10^9/L | 208 x10^9/L | |||
| MPV | 12.3 fL | 11.8 f | |||
| Urea | 8.30 mmol/L | 6.30 mmol/L | |||
| Creatinine | 84 umol/L | 91 umol/L | |||
| eGFR | 90.74 ml/min/1.73m2 | 82.73 ml/min/1.73m2 | |||
| Bilirubin | 6.60 umol/L | 12.00 umol/L | |||
| ALP | 70.00 IU/L | 73.00 IU/L | |||
| ALT | 20.60 IU/L | 17.90 IU/L | |||
| GGT | 14.00 IU/L | 12.00 IU/L | |||
| Total Protein | 68.30 g/L | 67.30 g/L | |||
| Albumin | 46.90 g/L | 39.90 g/L | |||
| Globulin | 21.4 g/L | 27.4 g/L | |||
| SHBG | 65.10 nmol/L | 53.80 nmol/L | |||
| HbA1c | 29.6 mmol/mol | 30.94 mmol/mol | |||
| Total Cholesterol | 6.37 mmol/L | 5.62 mmol/L | |||
| LDL Cholesterol | 3.75 mmol/L | 3.06 mmol/L | |||
| Non HDL Cholesterol | 4.13 mmol/L | 3.24 mmol/L | |||
| HDL Cholesterol | 2.24 mmol/L | 2.38 mmol/L | |||
| Triglycerides | 0.84 mmol/L | 0.40 mmol/L | |||
| CRP HS | 0.65 mg/L | 0.56 mg/L | |||
| Uric Acid | 281.00 umol/L | 261.00 umol/L | |||
| Creatine Kinase | 101.00 IU/L | 72.00 IU/L | |||
| Iron | 19.69 umol/L | 25.51 umol/L | |||
| TIBC | 68.19 umol/L | 63.01 umol/L | |||
| UIBC | 48.50 umol/L | 37.50 umol/L | |||
| Transferrin Saturation | 28.88% | 40.49 % | |||
| Ferritin | 25.50 ug/L | 46.90 ug/L | |||
| TSH | 2.93 mIU/L | 2.01 mIU/L | |||
| Free T3 | 3.61 pmol/L | 4.19 pmol/L | |||
| Free Thyroxine | 15.400 pmol/L | 17.200 pmol/L | |||
| FSH | 9.65 IU/L | 9.30 IU/L | |||
| LH | 4.61 IU/L | 4.84 IU/L | |||
| Oestradiol | 99.20 pmol/L | 88.90 pmol/L | |||
| Testosterone | 25.60 nmol/L | 19.30 nmol/L | |||
| Prolactin | 261.00 mU/L | 351.00 mU/L | |||
| DHEA Sulphate | 5.420 umol/L | 6.150 umol/L | |||
| Cortisol | 587.000 nmol/L | 675.000 nmol/L | |||
| Thyroglobulin Antibodies | <10 kIU/L | <10 kIU/L | |||
| Thyroid Peroxidase Antibodies | <9.0 kIU/ | <9.0 kIU/L | |||
| Lipoprotein A | 105 nmol/L | 109 nmol/L |
To give more context to this results:
My cholesterol markers markedly improved. A few years ago I ate a very high fat high calories diet for a few months, and since then my cholesterol has been very high. It has been very slowly going done, but the procedure seems to have had a positive effect, with a marked drop in total and LDL cholesterol, a slight increase in HDL and a more than 50% drop in triglycerides level.
My inflammation (hsCRP) got a bit better.
Iron markers increased substantially, but this is because I used to donate blood every 3 months and last time was September 14, 2020. So the change has very likely nothing to do with the procedure, it is just a normalization.
In conclusion, what I can say is that the results are equivocal. The Levine phenoblood age clock got worse (by about 4 years), almost all of which driven by a huge drop in serum albumin. Other areas improved, but often these was a continuation of existing trends. Overall the results got better, but at this point I can not say that this procedure induces rejuvenation. There can be many interpretations. Maybe I am too young and healthy. Maybe using membrane filtration rather than centrifugal force causes some issues (it is certainly the opinion of the russian biohackers who had a similar trial recently). Maybe I am not measuring the correct endpoints. But whatever it is, once I will have received the epigenetic age result, I intend to repeat the procedure a second time, with maybe an adjusted protocol based on information I am gathering. Or I may start directly with more aged volunteers.
To be continued.