Rejuvenation by dilution of plasma – Part 2 – Let’s clear that blood!

In Part 1, I described an experiment I am starting, first on myself in the spirit of self-experimentation, then on other volunteers. Before having my first session of plasma dilution, I got a bunch of markers measured to evaluate my condition before. Here is myself in data:

Sample dateBiomarkerValue
12/05/1986SexMale
17/12/2020Chronological age34 years old
15/06/2020Epigenetic age (mydnage.com, derivated from Horvath Original 2013)30 years old
17/12/2020Epigenetic age (mydnage.com, derivated from Horvath Original 2013)Waiting for data
08/12/2020Levine’s Phenotypical age clock (blood)22 years old
08/12/2020Haemoglobin143 g/L
08/12/2020Haematocrit0.416 L/L
08/12/2020Red Cell Count4.57 x10^12/L
08/12/2020MCV91.2 fl
08/12/2020MCH31.3 pg
08/12/2020MCHC343 g/L
08/12/2020RDW13.2%
08/12/2020White Cell Count4.51 x10^9/L
08/12/2020Neutrophils1.34 x10^9/L
08/12/2020Lymphocytes2.69 x10^9/L
08/12/2020Monocytes0.29 x10^9/L
08/12/2020Eosinophils0.06 x10^9/L
08/12/2020Basophils0.11 x10^9/L
08/12/2020Platelet Count191 x10^9/L
08/12/2020MPV12.3 fL
25/11/2020Urea8.30 mmol/L
25/11/2020Creatinine84 umol/L
25/11/2020eGFR90.74 ml/min/1.73m2
25/11/2020Bilirubin6.60 umol/L
25/11/2020ALP70.00 IU/L
25/11/2020ALT20.60 IU/L
25/11/2020GGT14.00 IU/L
25/11/2020Total Protein68.30 g/L
25/11/2020Albumin46.90 g/L
25/11/2020Globulin21.4 g/L
25/11/2020SHBG65.10 nmol/L
25/11/2020HbA1c29.6 mmol/mol
25/11/2020Total Cholesterol6.37 mmol/L
25/11/2020LDL Cholesterol3.75 mmol/L
25/11/2020Non HDL Cholesterol4.13 mmol/L
25/11/2020HDL Cholesterol2.24 mmol/L
25/11/2020Triglycerides0.84 mmol/L
25/11/2020CRP HS0.65 mg/L
25/11/2020Uric Acid281.00 umol/L
25/11/2020Creatine Kinase101.00 IU/L
25/11/2020Iron19.69 umol/L
25/11/2020TIBC68.19 umol/L
25/11/2020UIBC48.50 umol/L
25/11/2020Transferrin Saturation28.88%
25/11/2020Ferritin25.50 ug/L
25/11/2020TSH2.93 mIU/L
25/11/2020Free T33.61 pmol/L
25/11/2020Free Thyroxine15.400 pmol/L
25/11/2020FSH9.65 IU/L
25/11/2020LH4.61 IU/L
25/11/2020Oestradiol99.20 pmol/L
25/11/2020Testosterone25.60 nmol/L
25/11/2020Prolactin261.00 mU/L
25/11/2020DHEA Sulphate5.420 umol/L
25/11/2020Cortisol587.000 nmol/L
25/11/2020Thyroglobulin Antibodies<10 kIU/L
25/11/2020Thyroid Peroxidase Antibodies<9.0 kIU/
25/11/2020Blood Urea Nitrogen23.52 mg/dL
25/11/2020Lipoprotein A105 nmol/L
Data data data

Now that we are ready, let’s go to the clinic.

Yes, it is a plastic surgery clinic! Posh!

I am pretty pleased with how it went. The doctor was interested in anti-aging research though not aware of the latest studies regarding plasma dilution. She has been doing this procedure for years and is very familiar with the risks and benefits. The regulations in France limit the quantity of plasma that can be replaced in one go to 800mL. However she found out that depending on the health of the patient, replacing double this dose is perfectly safe. Obviously she would never break the law, so she would never process more than 800mL, but if she did, hypothetically, she would remove a full 1600mL of plasma and replace it with a saline solution (NaCL 0.9%) and a few mL of Anticoagulant Citrate Dextrose-A as described in part 1.

The machine is a Hemofenix, russian made, but she was not able to give me the exact pore size, only that it was at most 80-100 nm. She will try to get the exact pore sizes.

A 2-way catheter

My estimated plasma volume is 2630 mL according to this calculator  and others. So I would hypothetically have had more than 60% of my plasma processed, though by the nature of the dilution process that would amount more to 50%. Pretty close to the study I’m trying to replicate!

The whole process took 75 minutes from start to finish.

I was able to keep the extracted plasma with me, to hopefully have more tests run on it.

As far as how I feel, there aren’t many sensations. You feel a few tingling sensations here and there, you’re a little bit tired for the rest of the day, but overall it’s pretty painless and uneventful. Which is good.

During the dilution, I chatted a bit with the doctor and she gave me a few very intriguing anecdotes: this 87 years old professor in Russia whose having wonderful results doing the procedure every month, this guy with a metastasized cancer having been “cured” with one 4-sessions procedure, herself and lot of other aged individuals getting a lot more energy after 1 procedure for months or years, and even this doctor in Spain having treated alzheimer patients for 6 years with very good results,… and so on and so on. This is just anecdotal data, and am not sure why there aren’t more published reports/studies if they are so good. But that’s why we’re here after all, aren’t we?

The next 2 sessions are on December 21 and 23, they will be run with the same protocol. One month after the last session, I will get new blood markers measured, as well as epigenetic age by mydnage.com.

Rejuvenation by dilution of plasma – Part 1 – Setting up an experiment

A study made by the Conboys team made a lot of noise a few months ago: Rejuvenation of three germ layers tissues by exchanging old blood plasma with saline-albumin . This is but one more evidence of a systemic regulation of the aging process by blood factors, but with the added and exciting bonus that merely removing “pro-aging” factors in the blood could have profound rejuvenation effects. This would be a game changer, allowing an greatly affordable and easily scalable anti-aging therapy to be developped.

Michael J. Conboy and Irina M. Conboy have started a company called IMYu to develop a commercial anti-aging therapy based on these latest findings, and they are keeping the details quite secret. Obviously they want to protect some sort of intellectual property, and considering that plasmapheresis has been approved and practiced around the world for decades, they have to hold on to some secrets or another. So when they declare publicly that it would be dangerous to try dilution of plasma for anti-aging yourself, or even that it would be unethical for doctors to try to replicate the experiment on volunteers, they are merely try to scare people and protect an ill-defined IP.

Unfortunately this means that we have to go by their published work to try and guess what works in plasma dilution, and ignore their attempt to muddy the water. At least until they publish more data on their human trials.

While we wait, there are a hundred thousands people dying of aging every day, so unlike them, I think the ethical thing to do is actually to try and advance public knowledge on the subject as much as as possible to allow people to make intelligent and informed decision and save lives that would be otherwise lost waiting for the approval of a bureaucracy that doesn’t recognize aging as a medical issue.

And it turns out that there are a few clinics are and there that offer a commercial treatment a plasmapheresis. For example, the only one in France is this one. On the description page of the procedure, they claim that it “detoxifies the blood”, helps with “aging”, “neuro-degenerative diseases including Alzheimer”, can help against “chronic fatigue”, “skin aging” and “hair loss”, etc… Sounds like typical crackpot sales pitch of the anti-aging marketplace. Except that now we have mice studies to back some versions of that up.

There are a few differences with the original study done on mice, which might make this flavor of the therapy completely ineffective for rejuvenation. As a quick reminder, in the study, 50% of platelet-rich-plasma was removed by centrifugation, and replaced with a saline-albumin solution. Here are the specifics of what the clinic offers:

  • Membrane plasmapheresis (mTPE) (the machines are Hemofenix), which is less efficient than centrifugal. I couldn’t get the pore size of the filters they use but from what I understand it will remove most but not the biggest proteins. The solution used for replacement is physiologic saline solution (0.9% NaCl), with a few mL of Anticoagulant Citrate Dextrose-A as needed by the machine to operate.
  • The procedure is done in 4 sessions separated by 48 hours minimum, during each of which you usually get 800ml of your plasma replaced (~30%). Note that because mTPE is less efficient, less of your plasma will be replaced than the volume of plasma extracted.

So I decided to use their service to test the rejuvenation effect of plasma dilution. I am starting with me as the first test subject, and depending on data will move on with others.

To evaluate the efficiency of the procedure, which is the whole point of this experiment, I will take measurements of various useful (and less useful) biomarkers. The first session is booked for December 17, 2020, and will be described in the next blog post, along with the first data gathered.

Edit from December 24, 2020: I was able to get a bit more information on the machine used for this procedure: